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Development of Flexible Dispense-Printed Electrochemical Immunosensor for Aflatoxin M1 Detection in Milk.

Authors
  • Abera, Biresaw Demelash1
  • Falco, Aniello2
  • Ibba, Pietro3
  • Cantarella, Giuseppe4
  • Petti, Luisa5
  • Lugli, Paolo6
  • 1 Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy. [email protected] , (Italy)
  • 2 Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy. [email protected] , (Italy)
  • 3 Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy. [email protected] , (Italy)
  • 4 Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy. [email protected] , (Italy)
  • 5 Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy. [email protected] , (Italy)
  • 6 Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy. [email protected] , (Italy)
Type
Published Article
Journal
Sensors
Publisher
MDPI AG
Publication Date
Sep 11, 2019
Volume
19
Issue
18
Identifiers
DOI: 10.3390/s19183912
PMID: 31514303
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Detection of mycotoxins, especially aflatoxin M1 (AFM1), in milk is crucial to be able to guarantee food quality and safety. In recent years, biosensors have been emerging as a fast, reliable and low-cost technique for the detection of this toxin. In this work, flexible biosensors were fabricated using dispense-printed electrodes, which were functionalized with single-walled carbon nanotubes (SWCNTs) and subsequently coated with specific antibodies to improve their sensitivity. Next, the immunosensor was tested for the detection of AFM1 in buffer solution and a spiked milk sample using a chronoamperometric technique. Results showed that the working range of the sensors was 0.01 µg/L at minimum and 1 µg/L at maximum in both buffer and spiked milk. The lower limit of detection of the SWCNT-functionalized sensor was 0.02 µg/L, which indicates an improved sensitivity compared to the sensors reported so far. The sensitivity and detection range were in accordance with the limitation values imposed by regulations on milk and its products. Therefore, considering the low fabrication cost, the ease of operation, and the rapid read-out, the use of this sensor could contribute to safeguarding consumers' health.

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