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Characterization of eight Y-STR loci and haplotypes in a Chinese Han population

Authors
  • Tang, Jian Pin1
  • Hou, Yi Ping1
  • Li, Ying Bi1
  • Wu, Jin1
  • Zhang, Jian2
  • Zhang, Hai Jun3
  • 1 West China University of Medical Sciences, Institute of Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041, P.R. China , Chengdu
  • 2 Center of Forensic Sciences Service of Shandong Province, Jinan, Shandong, 250000, P.R. China , Jinan
  • 3 Public Security Bureau of Sichuan Province, Center of Forensic Sciences, Chengdu, Sichuan, 610041, P.R. China , Chengdu
Type
Published Article
Journal
International Journal of Legal Medicine
Publisher
Springer-Verlag
Publication Date
Jul 30, 2003
Volume
117
Issue
5
Pages
263–270
Identifiers
DOI: 10.1007/s00414-003-0371-x
Source
Springer Nature
Keywords
License
Yellow

Abstract

In this study we analyzed the eight Y-STR loci, DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458, investigated haplotype distributions of these Y-STR loci in a Chinese Han population, and sequenced alleles of the eight loci for clarifying the structure. Extracted DNA was amplified by PCR and the PCR products were analyzed by non-denaturing horizontal polyacrylamide gel electrophoresis with a discontinuous buffer system. Alleles were sequenced on an ABI 3700 using a Dye Terminator Cycle sequencing kit. DYS443, DYS453, DYS455 and DYS456 were found to be simple repeat systems, while DYS444, DYS448, DYS457 (DYS437) and DYS458 were complex repeat systems. The gene diversities of DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458 were 0.7742, 0.7671, 0.7453, 0.3545, 0.0549, 0.6988, 0.6148 and 0.8213, respectively. The haplotype diversity for 8 Y-STR loci was 0.9996, and the discrimination capacity was 0.9815. The results indicate that these eight loci are useful Y-linked markers for forensic applications.

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