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Characterization and biological activity of the main flavonoids from Swiss Chard (Beta vulgaris subspecies cycla).

Authors
  • Ninfali, P
  • Bacchiocca, M
  • Antonelli, A
  • Biagiotti, E
  • Di Gioacchino, A M
  • Piccoli, G
  • Stocchi, V
  • Brandi, G
Type
Published Article
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
Publication Date
Feb 01, 2007
Volume
14
Issue
2-3
Pages
216–221
Identifiers
PMID: 16698256
Source
Medline
License
Unknown

Abstract

The molecular components of a phenolic fraction (P2), obtained from liquid chromatography of a Swiss Chard (Beta vulgaris subsp. cycla) extract, were identified using HPLC-ESI-MS/MS. The primary P2 components were: vitexin-2''O-rhamnoside, its demethylated form 2''-xylosylvitexin, isorhamnetin 3-gentiobioside, and rutin. P2 "in toto" and the single components were characterized for antioxidant capacity, antimitotic activity on MCF-7 human breast cancer cells and for toxicity to human lymphocytes and macrophages. P2 inhibited MCF-7 cell proliferation (IC(50) value = 9 microg/ml) without inducing apoptosis, showed no toxicity to human lymphocytes and slight toxicity to macrophages. Vitexin-2''O-rhamnoside strongly inhibited DNA synthesis in MCF-7 cells, whereas 2''-xylosylvitexin and isorhamnetin 3-gentiobioside were activators; combinations of activators and inhibitors maintained the over-all inhibitory effect.

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