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Characterization of antibody-antigen interactions using biolayer interferometry

Authors
  • Noy-Porat, Tal1
  • Alcalay, Ron1
  • Mechaly, Adva1
  • Peretz, Eldar1
  • Makdasi, Efi1
  • Rosenfeld, Ronit1
  • Mazor, Ohad1
  • 1 Israel Institute for Biological Research, Ness-Ziona 7410001, Israel
Type
Published Article
Journal
STAR Protocols
Publisher
Elsevier
Publication Date
Sep 15, 2021
Volume
2
Issue
4
Identifiers
DOI: 10.1016/j.xpro.2021.100836
PMID: 34568849
PMCID: PMC8449132
Source
PubMed Central
Keywords
Disciplines
  • Protocol
License
Unknown

Abstract

This protocol describes the use of a biolayer interferometry platform for assessing antibody-antigen interactions. The protocol focuses on affinity determination and epitope binning, although the system can be utilized for measuring any protein-protein interaction. Readings are collected in real time, allowing the use of unlabeled molecules, and data can thus be obtained in a fast and easy manner. Experiments should be carefully designed, taking into consideration the tested interaction, available sensors, and suitable controls.

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