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Characterization of alphabaculovirus: HearNPV-IIPR05 isolate infecting Helicoverpa armigera (Hubner) larvae

Authors
  • Bandi, Sanjay M.1
  • Shanmugavadivel, P. S.1
  • Kumar, Lalit2
  • Revanasidda, A.1
  • 1 ICAR-Indian Institute of Pulses Research, Kanpur, 208024, India , Kanpur (India)
  • 2 ICAR-Indian Institute of Farming Systems Research, Modipuram, 250110, India , Modipuram (India)
Type
Published Article
Journal
Egyptian Journal of Biological Pest Control
Publisher
Springer Berlin Heidelberg
Publication Date
Jan 15, 2021
Volume
31
Issue
1
Identifiers
DOI: 10.1186/s41938-021-00367-9
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundThe alphabaculoviruses are lethal pathogens of lepidopteran caterpillars including a polyphagous and globally recognized pest, Helicoverpa armigera (Hubner) infesting economically important agriculture crops worldwide. The biological and molecular characterizations of indigenous nucleopolyhedrovirus of the genus Alphabaculovirus isolated from H. armigera in chickpea fields are described.ResultsThe virulence of virus isolate was tested in 3rd instar H. armigera larvae, and LC50 (median lethal concentration) was estimated to be 2.69 × 104 OBs ml−1. The ST50 (median survival time) was 4 days post-inoculation, when the 3rd instar H. armigera larvae were inoculated by OB (occlusion body) concentration equivalent to LC90. An average incubation period of the virus isolate in 3rd instar ranged between 4 and 6 days post-inoculation. The OBs of a virus isolate appeared irregular in shape and variable in size with diameter ranging from 0.57 to 1.46 μm on the longest edge and average of 1.071 ± 0.068 μm (mean ± SE). On the basis of phylogenetic analysis of polh, pif-1, and lef-8 genes, the isolate was found to be a member of the genus Alphabaculovirus. The isolate showed a genetic affinity with species of group II Alphabaculoviruses and appeared to be a group II NPV.ConclusionsOn the basis of molecular phylogeny and associated host insect, this indigenous isolate was designated as HearNPV-IIPR05 isolate, which could be a potential candidate for the biological control of H. armigera infesting legumes and other commercial crops.

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