Affordable Access

deepdyve-link
Publisher Website

Characteristics of regulatory T-cell populations before and after Ty21a typhoid vaccination in children and adults.

Authors
  • Rudolph, Mark E1
  • McArthur, Monica A2
  • Magder, Laurence S3
  • Barnes, Robin S4
  • Chen, Wilbur H5
  • Sztein, Marcelo B6
  • 1 Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA; Molecular Microbiology and Immunology Department, University of Maryland Graduate Program in Life Sciences, Baltimore, MD, USA. Electronic address: [email protected]
  • 2 Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA.
  • 3 Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore, MD, USA.
  • 4 Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.
  • 5 Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA; Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
  • 6 Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA; Molecular Microbiology and Immunology Department, University of Maryland Graduate Program in Life Sciences, Baltimore, MD, USA; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA; Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. Electronic address: [email protected]
Type
Published Article
Journal
Clinical Immunology
Publisher
Elsevier
Publication Date
Jun 01, 2019
Volume
203
Pages
14–22
Identifiers
DOI: 10.1016/j.clim.2019.04.002
PMID: 30953793
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Typhoid fever, caused by the pathogen Salmonella enterica serovar Typhi (S. Typhi), is a serious global health concern. Challenge studies with wild type S. Typhi identified associations between gut-homing regulatory T cells (Treg) and development of typhoid disease. Whether oral live-attenuated Ty21a vaccination induces gut-homing Treg remains unclear. Here, we analyze pediatric and adult Treg pre- and post-Ty21a vaccination in an autologous S. Typhi-antigen presentation model to address this knowledge gap. We show that peripheral memory Treg populations change from childhood to adulthood, but not following Ty21a vaccination. Unsupervised dimensionality reduction with t-distributed stochastic neighbor embedding (tSNE) identifies homing, memory, and functional features which evidence age-associated maturation of multifunctional S. Typhi-responsive Treg, which were not impacted by Ty21a vaccination. These findings improve understanding of pediatric regulatory T cells, while identifying age-related differences in S. Typhi-responsive Treg, which may aid in the development of improved pediatric vaccination strategies against S. Typhi. Copyright © 2019 Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times