Affordable Access

deepdyve-link
Publisher Website

Characteristics of Nasal Septal Cartilage-Derived Progenitor Cells during Prolonged Cultivation.

Authors
  • Kim, Do Hyun1
  • Lim, Jung Yeon1
  • Kim, Sung Won1
  • Lee, WeonSun2
  • Park, Sang Hi2
  • Kwon, Mi Yeon2
  • Park, Sun Hwa1
  • Lim, Mi Hyun1
  • Back, Sang A1
  • Yun, Byeong Gon1
  • Jeun, Jung Ho1
  • Hwang, Se Hwan3
  • 1 1 Department of Otolaryngology-Head and Neck Surgery, Seoul St Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. , (North Korea)
  • 2 2 Institute of Clinical Medicine Research, College of Medicine, The Catholic University of Korea, Seoul, Korea. , (North Korea)
  • 3 3 Department of Otolaryngology-Head and Neck Surgery, Bucheon St Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. , (North Korea)
Type
Published Article
Journal
Otolaryngology
Publisher
SAGE Publications
Publication Date
Oct 01, 2018
Volume
159
Issue
4
Pages
774–782
Identifiers
DOI: 10.1177/0194599818777195
PMID: 29787348
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Objective To produce alternate cell sources for tissue regeneration, human nasal septal cartilage-derived progenitor cells (NSPs) were tested to identify whether these cells meet the criteria of cartilage progenitor cells. We also evaluated the effects of prolonged cultivation on the characteristics of NSPs. Study Design In vitro study. Setting Academic research laboratory. Methods NSPs were isolated from discarded human nasal septal cartilage. NSPs were cultured for 10 passages. The expression of septal progenitor cell surface markers was assessed by fluorescence-activated cell sorting. Cell proliferation was measured with a cell-counting kit. Cytokine secretion was analyzed with multiplex immunoassays. Chondrogenic differentiation of NSPs without differentiation induction was analyzed with type II collagen immunohistochemistry. Cartilage-specific protein expression was evaluated by Western blotting. Under osteo- and adipodifferentiation media, 2 lineage differentiation potentials were evaluated by histology and gene expression analysis. Results Surface epitope analysis revealed that NSPs are positive for mesenchymal stem cells markers and negative for hematopoietic cell markers. Cultured NSPs showed sufficient cell expansion and chondrogenic potential, as demonstrated by immunostaining and expression of cartilage-specific protein. IL-6, IL-8, and transforming growth factor ß were secreted by over 200 pg/mL. The osteo- and adipodifferentiation potentials of NSPs were identified by histology and specific gene expression. The aforementioned characteristics were not influenced by prolonged cultivation. Conclusion NSPs represent an initial step toward creating a cell source from surgically discarded tissue that may prove useful in cartilage regeneration.

Report this publication

Statistics

Seen <100 times