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The characteristics of DNA methylation in an in vitro DNA synthesizing system from mouse fibroblasts.

Authors
  • Grafstrom, R H
  • Yuan, R
  • Hamilton, D L
Type
Published Article
Journal
Nucleic acids research
Publication Date
Apr 25, 1985
Volume
13
Issue
8
Pages
2827–2842
Identifiers
PMID: 4000965
Source
Medline
License
Unknown

Abstract

An in vitro DNA synthesizing system from mouse fibroblasts has been used to study DNA methylation. DNA methylation occurs in two phases, one at the replication fork and the other farther behind it. Although 4% of the dCMP residues in mouse cell DNA are mdCMP, only 1.7% of the total [alpha 32P]dCMP in newly replicated DNA is methylated in vitro. No methylation of Okazaki fragments was detected. Nearest neighbor analysis of the newly replicated DNA revealed that, although 40% of the CpG dinucleotides were methylated, significant amounts of cytosine methylation were also found in CpC, CpT, and CpA dinucleotides.

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