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The characteristics of the cell-free translation of mRNA from cells infected with the herpes virus pseudorabies virus.

Authors
  • McGrath, B M
  • Stevely, W S
Type
Published Article
Journal
The Journal of general virology
Publication Date
Aug 01, 1980
Volume
49
Issue
2
Pages
323–332
Identifiers
PMID: 6255071
Source
Medline
License
Unknown

Abstract

The translation in vitro of mRNA from pseudorabies virus infected cells was studied using systems derived from wheat germ and from rabbit reticulocyte. The mRNA was shown by molecular hybridization to contain sequences complementary to virus DNA. Products of in vitro translation co-migrating with virus proteins on polyacrylamide gel electropherograms were detected and the major immune precipitation. Optimum conditions for the stimulation of amino acid incorporation in vitro were determined and found to be similar for mRNA from both infected and mock-infected cells.

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