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Characteristic of Five Subpopulation Leukocytes in Single-Cell Levels Based on Partial Principal Component Analysis Coupled with Raman Spectroscopy.

Authors
  • Li, Wenxue1, 2
  • Wang, Liu3
  • Luo, Chuan1, 2
  • Zhu, Zhiqiang4
  • Ji, Jianlong5
  • Pang, Lin1
  • Huang, Qing2, 3
  • 1 College of Physics, Sichuan University, Chengdu, China. , (China)
  • 2 Department of Laboratory Medicine, Southwest Hospital, Chongqing, China. , (China)
  • 3 Department of Laboratory Medicine, Daping Hospital, Chongqing, China. , (China)
  • 4 College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, China. , (China)
  • 5 College of Information and Computer, Taiyuan University of Technology, Taiyuan, China. , (China)
Type
Published Article
Journal
Applied Spectroscopy
Publisher
SAGE Publications
Publication Date
Dec 01, 2020
Volume
74
Issue
12
Pages
1463–1472
Identifiers
DOI: 10.1177/0003702820938069
PMID: 32519582
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Characteristics of five subpopulation leukocytes in single-cell levels based on partial principal component analysis coupled with Raman spectroscopy were proposed to recognize the biochemical features of five subpopulation leukocytes. Using wavelet transform, the reconstructed spectra of the low-frequency wavelet coefficients were used to perform multiple principal component analysis based on segmented spectral data wreathing cover at 720-800 cm-1, 840-994 cm-1, and 1010-1070 cm-1 wavenumbers, respectively. Our approach is promising since it enables to establish a better understanding of the underlying molecular difference between the subtypes of leukocytes in a label-free manner and to estimate the source of infection.

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