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[Characterisation of human meniscus cells].

Authors
  • Hoberg, M1
  • Uzunmehmetoglu, G
  • Sabic, L
  • Reese, S
  • Aicher, W K
  • Rudert, M
Type
Published Article
Journal
Zeitschrift fur Orthopadie und ihre Grenzgebiete
Publication Date
Jan 01, 2006
Volume
144
Issue
2
Pages
172–178
Identifiers
PMID: 16625447
Source
Medline
Language
German
License
Unknown

Abstract

The meniscus of the human knee joint has an important function for the shock absorption, stability and power transmission from the upper to the lower leg. After meniscus injury often a partial or complete resection is necessary. Only injuries in the outer third may heal spontaneously or upon primary suture due to the vascularisation in these segments. After partial or total meniscectomy osteoarthritis of the knee joint is common in a large number of patients. The goal of our investigations was to establish meniscus cell cultures and to characterise the fibrochondrocytes (meniscus cells) in vitro. We examined the expression of different growth factors, cytokines and proteins in human menisci from surgical preparations using immunohistochemistry and RT-PCR analysis. Human meniscus cells express the collagens I , II, III, and VI, the matrix metalloproteinases-1, -2, -3, -8, and -13, BMP-2, and -4, TGFbeta1, VEGF, IGF-I, and -II, FGF-2, endostatin, iNOS, vimentin, TIMP-1, and -2, aggrecan, IL-1beta, IL-6, and IL-18. Staining with the monoclonal antibody AS.02 in all examined cells confirmed their mesenchymal origin. New strategies for the treatment of meniscus damage can be derived from these results and further advances for the tissue engineering of meniscus tissue can be obtained.

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