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Changes in some cytoskeletal proteins during neuroblastoma cell differentiation.

Authors
  • Edde, B
  • Portier, M M
  • Sahuquillo, C
  • Jeantet, C
  • Gros, F
Type
Published Article
Journal
Biochimie
Publisher
Elsevier
Publication Date
Feb 01, 1982
Volume
64
Issue
2
Pages
141–151
Identifiers
PMID: 7066411
Source
Medline
License
Unknown

Abstract

After in vitro microtubule assembly of mouse neuroblastoma crude extracts, six protein species migrate in the tubulin region of two-dimensional electrophoregrams. The evolution of these forms after morphological cell differentiation of the clone NIE115 shows two major modifications. Form 5 decreased drastically while form 6 increases during neurite formation. Peptide mapping analysis reveals that forms 5 and 6 are vimentin, a component of intermediate filaments, and beta-tubulin subunit, respectively. Sodium butyrate treatment of NIE115 cells or serum starvation of NIA103 cells, conditions blocking cell division and failing to induce morphological differentiation, prevent any modifications in the relative proportion of these proteins. It is concluded that the changes in the distribution of the tubulin isoforms and vimentin are directly related to neurite formation.

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