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Changes in the lipopolysaccharide of Proteus mirabilis 9B-m (O11a) clinical strain in response to planktonic or biofilm type of growth

Authors
  • Zabłotni, Agnieszka1
  • Matusiak, Dominik1
  • Arbatsky, Nikolay P.2
  • Moryl, Magdalena3
  • Maciejewska, Anna4
  • Kondakova, Anna N.2
  • Shashkov, Alexander S.2
  • Ługowski, Czesław4
  • Knirel, Yuriy A.2
  • Różalski, Antoni3
  • 1 University of Łódź, Laboratory of General Microbiology, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, Banacha 12/16, Łódź, 90-237, Poland , Łódź (Poland)
  • 2 Russian Academy of Sciences, N. D. Zelinsky Institute of Organic Chemistry, Leninsky Prospekt 47, Moscow, 119991, Russia , Moscow (Russia)
  • 3 University of Łódź, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, Banacha 12/16, Łódź, 90-237, Poland , Łódź (Poland)
  • 4 Polish Academy of Sciences, Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Rudolf Weigl 12, Wrocław, 53-114, Poland , Wrocław (Poland)
Type
Published Article
Journal
Medical Microbiology and Immunology
Publisher
Springer Berlin Heidelberg
Publication Date
Jan 12, 2018
Volume
207
Issue
2
Pages
129–139
Identifiers
DOI: 10.1007/s00430-018-0534-5
Source
Springer Nature
Keywords
License
Green

Abstract

The impact of planktonic and biofilm lifestyles of the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, and lipid A) was evaluated. Proteus mirabilis bacteria are able to form biofilm and lipopolysaccharide is one of the factors involved in the biofilm formation. Lipopolysaccharide was isolated from planktonic and biofilm cells of the investigated strain and analyzed by SDS–PAGE with silver staining, Western blotting and ELISA, as well as NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical and NMR spectroscopic analyses revealed that the structure of the O-polysaccharide of P. mirabilis 9B-m strain did not depend on the form of cell growth, but the full-length chains of the O-antigen were reduced when bacteria grew in biofilm. The study also revealed structural modifications of the core region in the lipopolysaccharide of biofilm-associated cells—peaks assigned to compounds absent in cells from the planktonic culture and not previously detected in any of the known Proteus core oligosaccharides. No differences in the lipid A structure were observed. In summary, our study demonstrated for the first time that changes in the lifestyle of P. mirabilis bacteria leads to the modifications of their important virulence factor—lipopolysaccharide.

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