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Changes of intermediate filaments in cultured human glioma cells with various growth factors and cytokines using high voltage immunoelectron microscopy.

Authors
Type
Published Article
Journal
Nōshuyō byōri = Brain tumor pathology
Publication Date
Volume
13
Issue
1
Pages
49–56
Identifiers
PMID: 8916126
Source
Medline
License
Unknown

Abstract

Morphological and immunocytological changes of intermediate filaments of cultured human malignant glioma cells were studied by adding various growth factors or cytokines using stereoscopic high voltage electron microscopy operated at 1,000 kV. The gold-colloid immuno-cytochemical method was used to stain GFAP and vimentin. Growth rate of tumor cells increased when EGF, TGF-alpha, and PDGF administered and decreased when FGF, TNF, and CLN-IgG administered. Morphological changes of cells were not remarkable when EGF, PDGF, IL-1, and FGF were administered. The cytoplalsmic organellaes were damaged after administrating TNF and CLN-IgG to cells.

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