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Changes in inflammatory factors in SV40MES13 mesangial cells after silencing ApoM gene

Authors
  • Liu, Jiongjiong1
  • Zhang, Yang2
  • Zhang, Xiao3
  • Zhang, Shuchang2
  • Hu, Yunfei2
  • Wang, Lizhuo2
  • Gao, Jialin2
  • Zhang, Yao2
  • 1 Department of International Medical Services, The First Affiliated Hospital of USTC-Divsion of Life Sciences and Medicine, University of Sciences and Technology of China, Hefei 230001, China
  • 2 Anhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College, Wuhu 241001, China
  • 3 Department of Pediatric Surgery, The First Affiliated Hospital of USTC-Divsion of Life Sciences and Medicine, University of Sciences and Technology of China, Hefei 230001, China
Type
Published Article
Journal
International journal of clinical and experimental pathology
Publication Date
Jun 01, 2020
Volume
13
Issue
6
Pages
1451–1456
Identifiers
PMID: 32661483
PMCID: PMC7344000
Source
PubMed Central
Keywords
License
Unknown

Abstract

Objective: Inflammation is an important process in the occurrence and development of nephropathy, and ApoM is closely related to inflammation. This article aims to investigate the inflammatory changes of SV40 MES13 cells after ApoM gene silencing by western blot and to explore the relationship between ApoM and inflammation. Methods: Control group glomerular mesangial cells (SV40 MES13), and the same cells after adding a small interfering RNA silencing ApoM gene for 24 h were observed under a microscope and photographed. After extracting the protein western blot was used to explore the associated inflammation of IL-6, P-Jak2, Erk, TNF-α, P-JNK, IKKβ, P-p38, IκBα, P-IKKα/β, NF-κB and P-NF-κB expression. Results: Western blot showed that ApoM gene was successfully silenced in SV40 MES13 cells after adding small interfering RNA. The decrease of inflammatory factors IL-6 and P-Jak2 in Jak/Stat pathway was statistically significant. Inflammatory cytokines TNF-α and P-JNK in the NF-κB pathway decreased statistically significantly, while the inflammatory factor IKKβ increased statistically significantly. Conclusion: Inflammation was suppressed in SV40 cells with ApoM gene silencing.

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