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[Changes in the dehydrogenase and GABA transaminase activity in the cerebral cortex during corazol kindling].

Authors
  • Kryzhanovskiĭ, G N
  • Moiseev, I N
  • Shandra, A A
  • Makul'kin, R F
  • Godlevskiĭ, L S
Type
Published Article
Journal
Biulleten' eksperimental'noĭ biologii i meditsiny
Publication Date
Jan 01, 1986
Volume
101
Issue
1
Pages
13–15
Identifiers
PMID: 3942808
Source
Medline
License
Unknown

Abstract

The experiments on (CBA X C57BL/6)F1 mice have shown that regular corazol injections in subliminal doses stimulated seizure susceptibility (pharmacological kindling). Cytophotometric assay of the activity of oxidative metabolism enzymes (glutamate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, alpha-oxoglutarate dehydrogenase, lactate dehydrogenase) and GABA-transaminase in the sensorimotor cortex of kindled mice in post-convulsive period, and 24 hours or 30 days after corazol injections were discontinued, has revealed some specific alterations of the enzymes under study, that suggest the existence of two phases of energy metabolism disturbances. The first phase (24 hours after corazol injections were discontinued) is characterized by intensified succinic acid oxidation, while the second phase (30 days after the last injection) is characterized by anaerobic glycolysis in neuronal and glial cells. Inhibition of GABA-transaminase activity was particularly marked in postconvulsive period. From a molecular point of view these data may be considered as enzyme disturbances during stimulation of seizure susceptability or seizure activity and as a compensation component ensuring anticonvulsive mechanisms and reparative processes (antagonistic principle of molecular mechanism regulation) during activation of antiepileptic system.

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