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Changes in the D1 receptor-adenylate cyclase complex after priming.

Authors
  • Morelli, M1
  • De Montis, G
  • Di Chiara, G
  • 1 Institute of Experimental Pharmacology and Toxicology, University of Cagliari, Italy. , (Italy)
Type
Published Article
Journal
European Journal of Pharmacology
Publisher
Elsevier
Publication Date
May 16, 1990
Volume
180
Issue
2-3
Pages
365–367
Identifiers
PMID: 2114301
Source
Medline
License
Unknown

Abstract

The D1 agonist, SKF 38393, failed to induce contralateral turning in drug-naive rats lesioned unilaterally with 6-hydroxydopamine from 17 days. Priming with a dopamine agonist, such as the D2 agonist, LY 171555, three days before, made SKF 38393 fully effective in inducing contralateral turning. Analysis of D1 receptor binding in striata of drug-naive and primed rats showed no change in the Bmax and Kd. In contrast, dopamine-stimulated adenylate cyclase showed a decrease in its Km for dopamine in the lesioned side of primed rats as compared with drug-naive rats. Thus, priming appears to elicit changes at the level of the transduction mechanism of D1 receptors rather than in the D1 recognition site itself.

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