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Changes in the circular dichroic spectrum of calf thymus solubilized chromatin caused by ultraviolet irradiation.

Authors
Type
Published Article
Journal
Radiation and environmental biophysics
Publication Date
Volume
16
Issue
2
Pages
117–123
Identifiers
PMID: 472114
Source
Medline

Abstract

UV irradiation of the chromatin caused an increase of the positive circular dichroic band in the vicinity of 275 nm (corresponding to DNA) and a deepening of the negative band of proteins at about 225 nm. These changes in the circular dichroic spectrum are monotonous in the range of doses studied (less than 6 X 10(4) J.m-2). The increase of the positive circular dichroic band probably reflects the occurrence of local conformational changes in DNA, which include changes in base position (tilting, distance from helix axis) in the close neighborhood of photoproducts. The presence of photoproducts in chromatin reduces changes in its circular dichroic spectra with temperature.

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