Affordable Access

Access to the full text

Challenges for Ex Situ Conservation of Wild Bananas: Seeds Collected in Papua New Guinea Have Variable Levels of Desiccation Tolerance

Authors
  • Kallow, Simon1, 2
  • Longin, Kevin1
  • Sleziak, Natalia Fanega
  • Janssens, Steven B.2, 3
  • Vandelook, Filip2
  • Dickie, John
  • Swennen, Rony1, 4
  • Paofa, Janet
  • Carpentier, Sebastien1,
  • Panis, Bart1,
  • 1 (B.P.)
  • 2 (F.V.)
  • 3 Plant Conservation and Population Biology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, 3001 Leuven, Belgium
  • 4 International Institute of Tropical Agriculture, c/o Nelson Mandela African Institution of Science and Technology, Arusha 0206, Tanzania
Type
Published Article
Journal
Plants
Publisher
MDPI AG
Publication Date
Sep 21, 2020
Volume
9
Issue
9
Identifiers
DOI: 10.3390/plants9091243
PMID: 32967145
PMCID: PMC7570212
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Green

Abstract

Ex situ seed conservation of banana crop wild relatives ( Musa spp. L.), is constrained by critical knowledge gaps in their storage and germination behaviour. Additionally, challenges in collecting seeds from wild populations impact the quality of seed collections. It is, therefore, crucial to evaluate the viability of seeds from such collecting missions in order to improve the value of future seed collections. We evaluate the seed viability of 37 accessions of seven Musa species, collected from wild populations in Papua New Guinea, during two collecting missions. Seeds from one mission had already been stored in conventional storage (dried for four months at 15% relative humidity, 20 °C and stored for two months at 15% relative humdity, −20 °C), so a post-storage test was carried out. Seeds from the second mission were assessed freshly extracted and following desiccation. We used embryo rescue techniques to overcome the barrier of germinating in vivo Musa seeds. Seeds from the first mission had low viability (19 ± 27% mean and standard deviation) after storage for two months at 15% relative humidity and −20 °C. Musa balbisiana Colla seeds had significantly higher post-storage germination than other species ( p < 0.01). Desiccation reduced germination of the seeds from the second collecting mission, from 84 ± 22% (at 16.7 ± 2.4% moisture content) to 36 ± 30% (at 2.4 ± 0.8% moisture content). There was considerable variation between and (to a lesser extent) within accessions, a proportion of individual seeds of all but one species ( Musa ingens N.W.Simmonds) survived desiccation and sub-zero temperature storage. We identified that seeds from the basal end of the infructescence were less likely to be viable after storage ( p < 0.001); and made morphological observations that identify seeds and infructescences with higher viability in relation to their developmental maturity. We highlight the need for research into seed eco-physiology of crop wild relatives in order to improve future collecting missions.

Report this publication

Statistics

Seen <100 times