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HeLa cells incubated with styryl compound C134

Authors
Publication Date
Keywords
  • Hela Cells
  • Styryl Compound
  • Hoechst Dye
Disciplines
  • Ecology
  • Geography
  • Mathematics

Abstract

This is part of a larger collection of archives of 1344 styryl compounds containing digital microscopic images of HeLa cells incubated with styryl compounds, along with other information about those compounds. ** The compounds were synthesized in a combinatorial synthesis involving 8 pyridinium/quinolinium groups (A-H) and 168 aldehyde groups (1-168). The compounds are identified by their A-H letter followed by their 1-168 number. ** Each of the 12 images in this archive is a 512x512 pixel image stored as a bzip2 compressed sequence of 2 byte unsigned short integers (little endian format), in standard raster order. ** Fluorescence in the Hoechst channel derives from Hoechst dye. Fluorescence in the other channels is presumed to derive from the styryl molecules, although cellular autofluorescence is also present. ** The images were acquired at 200X magnification with a 20X objective. The images were acquired with a 12 bit CCD camera so the greyscale intensities range from 0 to 4095. ** The "withdye" images were acquired after around 1 hour of incubation with the styryl probe. Next the probe was washed out using an onboard robotic pipetting system, and the "washout" images were acquired. ** The Hoechst dye images were acquired at 50msec exposure time, the other channels were acquired at both a 1sec and 200msec exposure time (Cy5 was only acquired at 1sec). ** The images were obtained using a Cellomics KineticScan instrument. Cells were kept alive and healthy in an onboard environmental control chamber. ** Styryl compounds were present at 100 micromolar concentration.

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