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P1,P4-Diadenosine 5′-Tetraphosphate Modulatesl-Arginine andl-Citrulline Uptake by Bovine Aortic Endothelial Cells

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
375
Issue
1
Identifiers
DOI: 10.1006/abbi.1999.1643
Keywords
  • Endothelial Cells
  • Ap4A
  • Transport
  • Arginine
  • Citrulline

Abstract

Abstract We have previously demonstrated that P1,P4-diadenosine 5′-tetraphosphate (Ap4A) interacts with high-affinity and low-affinity binding sites on the bovine aortic endothelial cell (BAEC) surface. In this report we demonstrate that Ap4A interaction with the lower affinity site modulates l-arginine (l-Arg) and l-citrulline (l-Cit) uptake by BAEC. Competition uptake studies demonstrate that l-Arg and l-Cit uptake occurs through a common transporter system that is sensitive to Ap4A. Evidence is also presented that is consistent with Ap4A modulating l-Arg uptake by increasing the affinity of l-Arg for the transporter.

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