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Cerebellar Regional Dissection for Molecular Analysis

Authors
  • Hamel, Katherine A.1
  • Cvetanovic, Marija1
  • 1 Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA
Type
Published Article
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Publication Date
Dec 05, 2020
Issue
166
Identifiers
DOI: 10.3791/61922
PMID: 33346205
PMCID: PMC7871298
Source
PubMed Central
Keywords
License
Unknown

Abstract

Different cerebellar regions have been implicated to play a role in distinct behavioral outputs, yet the underlying molecular mechanisms remain unknown. This work describes a method to reproducibly and quickly dissect cerebellar cortex of the hemispheres, anterior and posterior regions of the vermis, and the deep cerebellar nuclei in order to probe for molecular differences by isolating RNA and testing for differences in gene expression.

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