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Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

Authors
  • Farea, Manal1
  • Husein, Adam1
  • Halim, Ahmad Sukari2
  • Berahim, Zurairah1
  • Nurul, Asma Abdullah3
  • Mokhtar, Khairani Idah4
  • Mokhtar, Kasmawati1
  • 1 Universiti Sains Malaysia, Conservative Department, School of Dental Sciences, Kubang Kerian, Kelantan, 16150, Malaysia , Kubang Kerian (Malaysia)
  • 2 Universiti Sains Malaysia, Reconstructive Sciences Unit, School of Medical Sciences, Kubang Kerian, Kelantan, 16150, Malaysia , Kubang Kerian (Malaysia)
  • 3 Universiti Sains Malaysia, School of Health Sciences, Kubang Kerian, Kelantan, 16150, Malaysia , Kubang Kerian (Malaysia)
  • 4 Kulliyah of Dentistry, Department of Oral Biology, IIUM Kuantan Campus, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, Kuantan Pahang DM, 25200, Malaysia , Bandar Indera Mahkota (Malaysia)
Type
Published Article
Journal
Clinical Oral Investigations
Publisher
Springer-Verlag
Publication Date
Sep 22, 2015
Volume
20
Issue
6
Pages
1181–1191
Identifiers
DOI: 10.1007/s00784-015-1601-6
Source
Springer Nature
Keywords
License
Yellow

Abstract

ObjectivesThe purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).Materials and methodsSHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.ResultsTGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).ConclusionsERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1.Clinical relevanceThe cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.

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