Affordable Access

Access to the full text

Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions

Authors
  • Torrent, Marc1, 2
  • Chalancon, Guilhem1
  • de Groot, Natalia S.1
  • Wuster, Arthur1
  • Babu, M. Madan1
  • 1 Laboratory of Molecular Biology, Medical Research Council, Francis Crick Avenue, CB2 0QH Cambridge, UK
  • 2 elona, 08193 Barcelona, Spain
Type
Published Article
Journal
Science Signaling
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
Sep 04, 2018
Volume
11
Issue
546
Identifiers
DOI: 10.1126/scisignal.aat6409
PMID: 30181241
PMCID: PMC6130803
Source
PubMed Central
License
Green

Abstract

Decoding the information in mRNA during protein synthesis relies on tRNA adaptors, the abundance of which can affect the decoding rate and translation efficiency. To determine whether cells alter tRNA abundance to selectively regulate protein expression, we quantified changes in the abundance of individual tRNAs at different time points in response to diverse stress conditions in Saccharomyces cerevisiae . We found that the tRNA pool was dynamic and rearranged in a manner that facilitated selective translation of stress-related transcripts. Through genomic analysis of multiple data sets, stochastic simulations, and experiments with designed sequences of proteins with identical amino acids but altered codon usage, we showed that changes in tRNA abundance affected protein expression independently of factors such as mRNA abundance. We suggest that cells alter their tRNA abundance to selectively affect the translation rates of specific transcripts to increase the amounts of required proteins under diverse stress conditions.

Report this publication

Statistics

Seen <100 times