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Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Authors
  • Dzamukova, Maria R.1
  • Naumenko, Ekaterina A.1
  • Rozhina, Elvira V.1
  • Trifonov, Alexander A.1
  • Fakhrullin, Rawil F.1
  • 1 Kazan Federal University, Bionanotechnology Lab, Institute of Fundamental Medicine and Biology, Kreml uramı 18, Kazan, 420008, Republic of Tatarstan, Russian Federation , Kazan (Russia)
Type
Published Article
Journal
Nano Research
Publisher
Tsinghua University Press
Publication Date
Aug 29, 2015
Volume
8
Issue
8
Pages
2515–2532
Identifiers
DOI: 10.1007/s12274-015-0759-1
Source
Springer Nature
Keywords
License
Yellow

Abstract

Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles (MNPs). Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions (room temperature and physiologic media). Microscopy (TEM, SEM, AFM, and enhanced dark-field imaging) revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar (two-cell layers) cell sheets and 3D multicellular spheroids.

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