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Cell-free synthesis of ornithine aminotransferase of rat liver.

Authors
  • Hayashi, H
  • Katunuma, N
  • Chiku, K
  • Endo, Y
  • Natori, Y
Type
Published Article
Journal
Journal of biochemistry
Publication Date
Oct 01, 1981
Volume
90
Issue
4
Pages
1229–1232
Identifiers
PMID: 7309717
Source
Medline
License
Unknown

Abstract

Mitochondrial ornithine aminotransferase was synthesized in a rabbit reticulocyte cell-free system using polysomal mRNA from rat liver. Upon analyzing the primary translation product by electrophoresis and fluorography, we found that ornithine aminotransferase is synthesized not as a large precursor but as one with its authentic size. Free ribosomes were found to be the main site of synthesis of ornithine aminotransferase.

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