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Cell death in Ustilago maydis: comparison with other fungi and the effect of metformin and curcumin on its chronological lifespan.

Authors
  • Soberanes-Gutiérrez, Cinthia V1, 2
  • León-Ramírez, Claudia2
  • Sánchez-Segura, Lino2
  • Cordero-Martínez, Emmanuel1
  • Vega-Arreguín, Julio C1
  • Ruiz-Herrera, José2
  • 1 Laboratorio de Ciencias Agrogenómicas, de la Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México, Blv. UNAM 2011, Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, León Gto., México.
  • 2 Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Km. 9.6 Libramiento Norte Carr. Irapuato-León 36824 Irapuato Gto., México.
Type
Published Article
Journal
FEMS Yeast Research
Publisher
Oxford University Press
Publication Date
Oct 15, 2020
Volume
20
Issue
7
Identifiers
DOI: 10.1093/femsyr/foaa051
PMID: 32945857
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Ustilago maydis is a Basidiomycota fungus, in which very little is known about its mechanisms of cell survival and death. To date, only the role of metacaspase1, acetate and hydrogen peroxide as inducers of cell death has been investigated. In the present work, we analyzed the lifespan of U. maydis compared with other species like Sporisorium reilianum, Saccharomyces cerevisiae and Yarrowia lipolytica, and we observed that U. maydis has a minor lifespan. We probe the addition of low concentrations metformin and curcumin to the culture media, and we observed that both prolonged the lifespan of U. maydis, a result observed for the first time in a phytopathogen fungus. However, higher concentrations of curcumin were toxic for the cells, and interestingly induced the yeast-to-mycelium dimorphic transition. The positive effect of metformin and curcumin appears to be related to an inhibition of the mechanistic Target of Rapamycin (mTOR) pathway, increase expression of autophagy genes and reducing of reactive oxygen species. These data indicate that U. maydis may be a eukaryotic model organism to elucidate the molecular mechanism underlying apoptotic and necrosis pathways, and the lifespan increase caused by metformin and curcumin. © The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.

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