Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry.
HSCI-CRM Flow Cytometry Core Facility, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts.
Stanford Shared FACS Facility, Stanford, California.
FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany.
Fred Hutchinson Cancer Research Center, Seattle, Washington.
Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts.
NYU Langone Medical Center, New York, New York.
- Published Article
Current protocols in cytometry
- Publication Date
Jan 01, 2019
Maintenance of hematopoietic stem cell (HSC) quiescence is critical for self-renewal and differentiation into mature lineages. Therefore, the ability to reliably detect abnormal HSC cycling is essential for experiments that seek to investigate abnormalities of HSC function. The ability to reproducibly evaluate cell cycle status in a rare cell subset requires careful optimization of multiple parameters during cell preparation and sample processing. Here, we describe a method where data acquisition parameters and fluorochrome combination for long-term HSC staining have been specifically designed for concurrent use with DAPI and Ki-67 antibodies. © 2018 by John Wiley & Sons, Inc. © 2018 John Wiley & Sons, Inc.
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This record was last updated on 10/23/2019 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/30335223