Affordable Access

Cell- and inducer-specific accretion of human isometallothioneins.

Authors
  • M Cavigelli
  • J H Kägi
  • P E Hunziker
Publication Date
Jun 01, 1993
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

The synthesis of metallothionein (MT) was investigated in three different human epithelial cell lines, each derived from one of the embryonic germ layers. The accretion of different isoforms of the protein was monitored using a sensitive neutral-pH h.p.l.c. method. Induction of MT synthesis by zinc ions and dexamethasone revealed differences between the three cell lines, both with respect to the number and the amounts of the different isoMTs formed. Dose-response experiments showed that an increase in dexamethasone concentration enhances MT accretion asymptotically to a limit, whereas within the concentration range explored zinc produces an exponential augmentation.

Report this publication

Statistics

Seen <100 times