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A Ceftazidime-Avibactam-Resistant and Carbapenem-Susceptible Klebsiella pneumoniae Strain Harboring bla KPC-14 Isolated in New York City.

Authors
  • Niu, Siqiang1
  • Chavda, Kalyan D2
  • Wei, Jie3
  • Zou, Chunhong4
  • Marshall, Steven H5
  • Dhawan, Puneet6
  • Wang, Deqiang4, 7
  • Bonomo, Robert A5, 8, 9
  • Kreiswirth, Barry N2
  • Chen, Liang10, 11
  • 1 Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China [email protected] [email protected] , (China)
  • 2 Hackensack Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USA. , (Jersey)
  • 3 Department of Laboratory Medicine, West China Second University Hospital, and Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Sichuan University, Chengdu, China. , (China)
  • 4 College of Laboratory Medicine, Chongqing Medical University, Yuzhong District, Chongqing, China. , (China)
  • 5 Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA.
  • 6 Molecular Resource Facility and Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA. , (Jersey)
  • 7 The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Yuzhong District, Chongqing, China. , (China)
  • 8 Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA.
  • 9 Case VA Center for Antimicrobial Resistance and Epidemiology (CARES), Cleveland, Ohio, USA.
  • 10 Hackensack Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USA [email protected] [email protected] , (Jersey)
  • 11 Department of Medical Sciences, Hackensack Meridian School of Medicine, Nutley, New Jersey, USA. , (Jersey)
Type
Published Article
Journal
mSphere
Publication Date
Aug 26, 2020
Volume
5
Issue
4
Identifiers
DOI: 10.1128/mSphere.00775-20
PMID: 32848008
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Ceftazidime-avibactam is a potent antibiotic combination against Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae Here, we describe a unique ceftazidime-avibactam-resistant and carbapenem-susceptible K. pneumoniae strain harboring a novel bla KPC-14 variant. This strain was isolated from a New York City patient in 2003, which predates the introduction of avibactam. Despite resistance to ceftazidime-avibactam, the strain was susceptible to imipenem-relebactam and meropenem-vaborbactam. Comprehensive genomic sequencing revealed that bla KPC-14 is harbored on an ST6 IncN plasmid associated with the early spread of bla KPC IMPORTANCE KPC is currently the most common carbapenemase identified in the United States. More than 40 KPC variants have been described, of which KPC-2 and KPC-3 are the most frequent clinical variants. However, our understanding of the genetic structures and β-lactam resistance profiles of other novel KPC variants remains incomplete. Here, we report a novel bla KPC variant (bla KPC-14) and the complete genome sequence of bla KPC-14-harboring K. pneumoniae strain BK13048, which is susceptible to carbapenems but resistant to ceftazidime-avibactam. To the best of our knowledge, this is one of the earliest KPC-producing K. pneumoniae strains exhibiting resistance to ceftazidime-avibactam. Copyright © 2020 Niu et al.

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