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Murine delayed hypersensitivity (DHS): Modulation ofin vitroantigen-induced responsiveness of lymph node cells from mice expressing DHS to human γ-globulin by lymphocyte populations from normal syngeneic animals

Authors
Journal
Cellular Immunology
0008-8749
Publisher
Elsevier
Publication Date
Volume
51
Issue
2
Identifiers
DOI: 10.1016/0008-8749(80)90261-0
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract Using a chemically defined, protein-free medium, the modulatory effect of normal (N) lymphocytes on in vitro antigen-induced proliferation by lymph node cells (LNC) from mice immunized to express delayed hypersensitivity (DHS) to human γ-glogulin (HGG) was quantitated in coculture. LNC from normal syngeneic animals exerted little if any effect on immune-LNC proliferation. Compared with immune-LNC plus N-LNC coculture response. N thymus cells (TC) were consistently suppressive while N spleen cells (SC) varied in their effect from a marginal to a marked potentiation of radiolabeled thymidine incorporation. Inactivation of N-SC suspensions by X irradiation prior to coculture with immune LNC abrogated the increased responsiveness. It therefore appeared that interaction of immune LNC and antigen resulted in recruitment of N-SC to proliferate. Separation of N-SC suspensions to provide enriched populations of thymic-independent (B) and thymic-dependent (T) lymphocytes showed that B cells augmented and T cells suppressed HGG-induced incorporation of [3H] thymidine when cocultured with immune LNC.

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