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Observations on the Effects of Condensates from Cigarette Smoke on Human Foetal Lung In Vitro

British Journal of Cancer
Nature Publishing Group
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547 OBSERVATIONS ON THE EFFECTS OF CONDENSATES FROM CIGARETTE SMOKE ON HUMAN FOETAL LUNG IN VITRO ILSE LASNITZKI* From the Strangeways Research Laboratory, Cambridge Received for publication September 23, 1958 IN previous experiments it was shown that 3-4-benzpyrene when added to the culture medium induced changes of a precancerous nature in organ cultures of human foetal lung. These consisted of hyperplasia of the bronchiolar epithelium with irregular increase in nuclear size, hyperchromatosis and abnormal cell divisions. The present work is an attempt to examine by the same method the effect of condensates from cigarette smoke on human foetal lung in culture. MATERIAL AND TECHNIQUE The smoke condensatest were kindly supplied by the late Sir Ernest Kennaway. All but one batch were derived from Denicotea filters and prepared by Mrs. G. Lewis, Mrs. M. Urquhart and Dr. J. M. Campbell, then all members of Sir Ernest Kennaway's Department at St. Bartholomew's Hospital, London, whose help in this investigation I gratefully acknowledge, while one batch was derived from a smoking machine. The neutral fractions of the condensate from which nicotine and phenols had been removed were used throughout the experiments. After evaporation of the solvent, either acetone or cyclohexane, they were freeze dried and homogenised with sterile horse serum. The freeze drying made the waxy condensates brittle and facilitated the homogenising process. The resulting suspension was kept at 40 C. and diluted to the desired concentration with human serum directly before use. Four variations of the neutral fraction were used: 1. The whole of the neutral fraction (solvent acetone) (a) from Denicotea filters, (b) from a smoking machine. 2. Neutral fraction from Denicotea ifiters (solvent cyclohexane). This contains approximately 90 per cent of the hydrocarbons present in the neutral fraction. 3. Neutral fraction from Denicotea filters, from which the hydrocarbons had been removed by column chromatography. Th

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