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The process of infection with bacteriophage φX174:XXVI. Transfer of the parental DNA of bacteriophage φX174 into progeny bacteriophage particles

Authors
Journal
Journal of Molecular Biology
0022-2836
Publisher
Elsevier
Publication Date
Volume
39
Issue
3
Identifiers
DOI: 10.1016/0022-2836(69)90150-8
Disciplines
  • Biology

Abstract

Abstract The experiments described in this paper show the following: (1) Parental DNA of bacteriophage φX174 appears as an intact molecule in a newly synthesized protein coat among the progeny phage particles. (2) This transferred parental DNA has to pass through a double-stranded state, the parental replicative form DNA, before it is released into progeny particles. (3) Cytoplasmic parental replicative form DNA rather than the membrane-associated parental replicative form DNA is the precursor for the transferred DNA. (4) Single-stranded φX DNA free of coat protein can be detected in the host cell when the infection has been started in the absence of DNA synthesis. Normally, the parental single-stranded virus DNA is rapidly converted to a double-stranded replicative form DNA. (5) The super-infection exclusion phenomenon in φX-infected cells is probably based on alterations in the host cell surface.

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