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A cDNA encoding a putative lipid transfer protein expressed in sunflower seeds.

Authors
  • Regente, Mariana
  • de la Canal, Laura
Type
Published Article
Journal
Journal of Plant Physiology
Publisher
Elsevier
Publication Date
Feb 01, 2003
Volume
160
Issue
2
Pages
201–203
Identifiers
PMID: 12685036
Source
Medline
License
Unknown

Abstract

Based on the N-terminal sequence of a sunflower antifungal protein, a full length cDNA (Ha-LTP5) encoding a putative lipid transfer protein from sunflower seeds was cloned using a RT-PCR based strategy. However, the sequence of the deduced protein is not identical to that of the antifungal protein previously isolated. The nucleotide sequence presents an ORF of 116 amino acids with a putative signal peptide, thus encoding a mature protein of 90 amino acids that is basic and hydrophobic. In contrast to the pattern of expression described for most LTP-like genes from dicots, Northern blot analyses detected constitutive expression of Ha-LTP5 in seeds, but not in aerial parts of sunflower plants.

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