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High Micromolar Ca2+beneath the Plasma Membrane in Stimulated Neutrophils

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
248
Issue
3
Identifiers
DOI: 10.1006/bbrc.1998.9031
Keywords
  • Ca2+Signalling
  • Neutrophils
  • Ffp-18
  • Membrane Ca2+

Abstract

Abstract Ca 2+near the inner face of the plasma membrane, as reported by the membrane associated fluorescent Ca 2+probe FFP-18, was higher than the bulk cytosolic free Ca 2+concentration both in resting neutrophils and in response to f-met-leu-phe. Influx caused Ca 2+close to the plasma membrane to rise more rapidly than the bulk cytosolic free Ca 2+and to reach a peak concentration of at least 30 μM. This zone of high Ca 2+was localised to just beneath the plasma membrane and did not extend more than 0.1 μm into the cell, as it was undetected by the bulk cytosolic free Ca 2+probes magfura2 and fura2. From these data, reconstruction of the distribution of Ca 2+within the neutrophil showed that the high Ca 2+signal at the cell cortex rapidly subsided to give a uniform free Ca 2+across the cell.

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