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Signaling pathways mediating the suppression ofArabidopsis thalianaKu gene expression by abscisic acid

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
1874-9399
Publisher
Elsevier
Publication Date
Volume
1779
Issue
3
Identifiers
DOI: 10.1016/j.bbagrm.2007.12.005
Keywords
  • Abscisic Acid
  • Dna End-Binding
  • Dna Double-Stranded Break
  • Gene Regulation
  • Phytohormone
  • Signal Transduction
Disciplines
  • Biology

Abstract

Abstract The Ku heterodimer facilitates the regulation of DNA repair, DNA replication, cell cycle, and telomere maintenance. The plant hormone abscisic acid (ABA) is a plant growth inhibitor. This study investigates how Arabidopsis thaliana Ku ( AtKu) genes are regulated by ABA in 3-week-old seedlings. First, β-Glucuronidase assay and real time quantitative PCR analysis results indicate that ABA represses the AtKu gene in a time- and concentration-dependent manner. However, adding of ABA biosynthesis inhibitors, fluride and tungate, did not eliminate AtKu suppression. Moreover, analysis of inhibitor treatments and ABA-responsive mutants suggested that AtKu repression by ABA was mediated through the pathway of extracellular Ca 2+, phospholipase D alpha, p38-type mitogen-activated protein kinase (MAPK), MAPK6 and ABA transcription factors, ABI3 and ABI5. Finally, no cross-talk in modulating AtKu gene expression existed between ABA and antagonist hormones (auxins and gibberellic acid).

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