Affordable Access

Publisher Website

CD8(+) tumor-infiltrating lymphocytes contribute to spontaneous "healing" in HER2-positive ductal carcinoma in situ.

  • Morita, Michi1, 2, 3
  • Yamaguchi, Rin1, 2
  • Tanaka, Maki3
  • Tse, Gary M4
  • Yamaguchi, Miki3
  • Kanomata, Naoki5
  • Naito, Yoshiki2
  • Akiba, Jun2
  • Hattori, Satoshi6
  • Minami, Shigeki7
  • Eguchi, Susumu8
  • Yano, Hirohisa2
  • 1 Department of Pathology and Laboratory Medicine, Kurume University Medical Center, Kurume, Fukuoka, Japan. , (Japan)
  • 2 Department of Pathology, Kurume University School of Medicine, Kurume, Fukuoka, Japan. , (Japan)
  • 3 Department of Surgery, Japan Community Health Care Organization Kurume General Hospital, Kurume, Fukuoka, Japan. , (Japan)
  • 4 Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong, Hong Kong. , (Hong Kong SAR China)
  • 5 Department of Pathology, Kawasaki Medical School, Kurashiki, Okayama, Japan. , (Japan)
  • 6 Department of Biostatistics Center, Kurume University School of Medicine, Kurume, Fukuoka, Japan. , (Japan)
  • 7 Department of Surgery, Nagasaki Harbor Medical Center City Hospital, Nagasaki, Japan. , (Japan)
  • 8 Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. , (Japan)
Published Article
Cancer Medicine
Publication Date
Jul 01, 2016
DOI: 10.1002/cam4.715
PMID: 27061242


We evaluated the associations between tumor-infiltrating lymphocytes (TIL) including CD8-positive [+] lymphocytes in ductal carcinoma in situ (DCIS) and histopathologic factors, particularly spontaneous "healing" and immunohistochemical (IHC)-based subtypes, to clarify the effects of host immune response to cancer cells proliferation during early carcinogenesis for the breast cancer. This cohort enrolled 82 DCIS patients. We examined the relationships between clinicopathologic factors including age, DCIS architecture, Van Nuys classification, grade, comedo necrosis, apocrine features, TIL, CD8(+) lymphocytes, healing, estrogen receptor and HER2 positivity, and IHC-based subtypes [luminal, luminal-HER2, HER2-positive, triple negative (TN)]. The results were analyzed by univariate and multivariate analyses. High numbers of TIL (high-TIL) and healing were seen in 30.5% and 39.0% of the cohort, respectively. The distributions of luminal, luminal-HER2, HER2 and TN subtypes were 73.2%, 9.8%, 13.4%, and 3.6%, respectively. High Van Nuys grading, high-grade, comedo necrosis, apocrine features, high-TIL, high CD8(+) lymphocytes and healing were significantly associated with HER2-positive (luminal-HER2, HER2), and TN subtypes. High-TIL was significantly associated with high-grade, comedo necrosis, apocrine features, healing, high CD8(+) lymphocytes and HER2 and TN subtypes. Healing was significantly correlated with high CD8(+) lymphocytes, high-grade, comedo necrosis, apocrine features, and HER2-positive and TN subtypes. Logistic regression analysis revealed a strong association between healing and TIL (odds ratio: 11.72, P = 0.024). High CD8(+) lymphocytes was also significantly associated with healing (odds ratio: 9.26, P = 0.009). The results of this study suggested that the spontaneous healing phenomenon might be induced by CD8(+) high-TIL associated with high-grade, comedo necrosis, apocrine features and HER2-positive DCIS. © 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

Report this publication


Seen <100 times