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Localization and quantitation of human superoxide dismutase using computerized 2-D gel electrophoresis

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
102
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(81)80185-4
Disciplines
  • Biology

Abstract

Summary The location of human superoxide dismutase has been identified on 2-D gels of cultured fibroblast extracts. Human superoxide dismutase was located on 2-D gels of a mouse-human somatic cell hybrid containing only human chromosome 21, and was absent in the mouse parental cell line. The native enzyme is a diner composed of two identical monomers present on 2-D gels as a single spot of molecular weight approximately 16,000 and isoelectric point of pH 6.2. Comparison of the density of the SOD spot on gels of a trisomy 21 cell strain to that of a monosomy 21 cell strain showed a 3 fold increase indicating agene dosage effect was present. This approach demonstrates the utility of quantitative 2-0 analysis for gene dosage studies.

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