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Anti-mitogenic effects of sarpogrelate in cultured rat mesangial cells

Authors
Journal
Life Sciences
0024-3205
Publisher
Elsevier
Publication Date
Volume
60
Issue
11
Identifiers
DOI: 10.1016/s0024-3205(97)00018-0
Keywords
  • Sarpogrelate
  • Serotonin
  • Dna Synthesis
  • Mesangial Cells
  • Cell Cycle

Abstract

Abstract We investigated the effects of the new 5-HT2A receptor antagonist, sarpogrelate, on DNA synthesis in renal mesangial cells stimulated with 5-HT in the presence and absence of platelet-derived growth factor (PDGF)-BB. Both 5-HT and PDGF-BB demonstrated a mitogenic effect on these cells. When mesangial cells were incubated in the absence of PDGF-BB, sarpogrelate inhibited DNA synthesis in these cells in a dose-depndent manner. In the presence of PDGF-BB, sarpogrelate had a weaker anti-mitogeniceffect in mesangialcells stimulated with 5-HT. Sarpogrelate was cytotoxic at concentrations over 10 −5 M according to the results of LDH release assays, and it reduced the S1 phase in mesangial cells stimulated with 5-HT by a flow cytometry. These findings suggest that sarpogrelate may be effective in the treatment of some glomerulonephritis associated with mesangialcell proliferation.

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