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Mitogen stimulation during rosette formation: A test to measure numbers and function of T, B, ‘D’ and ‘null’ lymphocytes in man

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
38
Identifiers
DOI: 10.1016/0022-1759(80)90279-3
Disciplines
  • Medicine

Abstract

Abstract A technically simple procedure is described for simultaneous determination of numbers and functions of human blood lymphocyte subpopulations. The method is based on stimulation with mitogens (PHA, Con A and PWM) with simultaneous rosetting to detect E receptors and C3 receptors. Con A and PWM activate the affinity of C3 receptors in normal lymphocytes increasing the number of cells rosetting with zymosan-complement particles, and also the number of cells giving mixed rosettes with both zymosan-complement particles and sheep red blood cells (B cells and ‘D’ cells respectively). PHA activates E receptors, increasing the number of T cells detected. The lymphocytes of 33 patients with allergic diseases of the skin and respiratory tract showed a decrease in C3 rosettes during stimulation with Con A and PWM, which suggests a defect of helper and suppressor functions of lymphocytes in these patients.

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