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A rapid method for the determination of guanosine 5′-diphosphate-3′-diphosphate and guanosine 5′-triphosphate-3′-diphosphate by high-performance liquid chromatography

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
121
Issue
1
Identifiers
DOI: 10.1016/0003-2697(82)90566-8

Abstract

Abstract A method is described for the rapid analysis of the nucleotides, guanosine 5′-diphosphate-3′-diphosphate (ppGpp) and guanosine 5′-triphosphate-3′-diphosphate (pppGpp), by high-performance liquid chromatography. It has been found that the inclusion of magnesium acetate in the potassium phosphate buffer facilitates elution of these highly phosphorylated compounds from the Partisil strong anion-exchange resin and allows their determination under isocratic conditions. The application of this methodology to formic acid extracts of bacterial cells is demonstrated.

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