Abstract The detection of veterinary drugs in blood meal is needed since it is used as an environment-friendly agricultural material despite its origination from animal blood. A method using accelerated solvent extraction and liquid chromatographic linear ion trap quadrupole Orbitrap mass spectrometry was developed to determine sedatives and adrenergic blockers in blood meal. The determination method was established following optimizations of accelerated solvent extraction, dispersive solid-phase extraction and high resolution mass spectrometric detection. Linearity, sensitivity, accuracy, repeatability and reproducibility of the method were fully validated. The method was applied to commercial blood meal products.