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Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis

Authors
Journal
Nano Letters
1530-6984
Publisher
American Chemical Society
Publication Date
Volume
12
Issue
3
Identifiers
DOI: 10.1021/nl203983e
Source
Legacy
Keywords
  • Letter

Abstract

We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40–56 nm, upon increasing cholesterol content from 0 to 20%.

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