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Two-dimensional gel electrophoresis as a taxonomic tool: Evidence from the centrospermae

Authors
Journal
Biochemical Systematics and Ecology
0305-1978
Publisher
Elsevier
Publication Date
Volume
17
Identifiers
DOI: 10.1016/0305-1978(89)90102-6
Keywords
  • Centrospermae
  • Chenopodiales
  • Caryophyllales
  • Systematics
  • Two-Dimensional Gel Electrophoresis
  • Cluster Analysis
  • Phenetics

Abstract

Abstract Two-dimensional polyacrylamide gel (2-D PAGE) electrophoresis was appraised as an experimental technique for assessing systematic relationships among higher plants and to determine at which level in the taxonomic hierarchy this technique is most generally applicable. 2-D PAGE was performed on denatured extracts of mature leaves from 25 species representing five families of the order Centrospermae (Caryophyllales, Chenopodiales) in the Angiospermae as well as Welwitschia mirabilis (Gymnospermae). Cluster analysis of a 256 spot binary-coded data set derived from the computer-encoded spot patterns of the 25 species successfully separated taxa from the individual to the familial levels of the taxonomic hierarchy in accordance with traditional taxonomic delineations of the taxa.

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