Affordable Access

Publisher Website

Separation of four isomers of lysergic acid α-hydroxyethylamide by liquid chromatography and their spectroscopic identification

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
236
Issue
2
Identifiers
DOI: 10.1016/s0021-9673(00)84895-5
Disciplines
  • Chemistry

Abstract

Abstract A method is described for the separation of four isomers of lysergic acid α-hydroxyethlamide and its decomposition products ergine and erginine using LiC-hrosorb NH 2 as the stationary phase and isocratic elution. The substances under study were determined by 13C and 1H NMR and mass spectroscopy. The relative proportions of individual isomers of lysergic acid α-hydroxyethylamide, ergine and erginine in the fermentation medium are assumed to result from chemical equilibrium reactions. The method is reproducible and suitable for kinetic studies of the isomerization and degradation of lysergic acid α-hydroxyethylamide.

There are no comments yet on this publication. Be the first to share your thoughts.