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Use of immobilizedBacillus subtilisfor the stereospecific hydrolysis ofd,l-menthyl acetate

Authors
Journal
Enzyme and Microbial Technology
0141-0229
Publisher
Elsevier
Publication Date
Volume
9
Issue
4
Identifiers
DOI: 10.1016/0141-0229(87)90018-4
Keywords
  • Bacillus Subtilis
  • Immobilized Systems
  • Menthyl Acetate

Abstract

Abstract Bacillus subtilis possessing a stereospecific menthyl esterase has been immobilized in a polyurethane foam and used for the hydrolysis of menthyl ester. The specific activity of cells immobilized in polyurethane foam decreased as the cell loading was increased. The aqueous content of the immobilized biocatalyst particles influenced the activity. The activity half-life of about 400 h observed for the immobilized biocatalyst is 66 × greater than that observed for free bacteria.

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