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Cavin3 Suppresses Breast Cancer Metastasis via Inhibiting AKT Pathway

  • An, Xin1, 2, 3
  • Lin, Xi1, 4
  • Yang, Anli1, 5
  • Jiang, Qiwei3
  • Geng, Bingchuan3
  • Huang, Mayan1, 6
  • Lu, Jiabin1, 6
  • Xiang, Zhicheng1
  • Yuan, Zhongyu1, 2
  • Wang, Shusen1, 2
  • Shi, Yanxia1, 2
  • Zhu, Hua3
  • 1 State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou , (China)
  • 2 Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou , (China)
  • 3 Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH , (United States)
  • 4 Departments of Ultrasound, Sun Yat-sen University Cancer Center, Guangzhou , (China)
  • 5 Department of Breast Oncology, Sun Yat-sen University Cancer Center, Guangzhou , (China)
  • 6 Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou , (China)
Published Article
Frontiers in Pharmacology
Frontiers Media SA
Publication Date
Sep 30, 2020
DOI: 10.3389/fphar.2020.01228
PMID: 33101009
PMCID: PMC7556234
PubMed Central


Objective Cavin3 is a putative tumor suppressor protein. However, its molecular action on tumor regulation is largely unknown. The aim of the current study is to explore the implication of cavin3 alteration, its clinical significance, and any potential molecular mechanisms in the regulation of breast cancer (BC). Methods TCGA (The Cancer Genome Atlas) and GTEx (Genotype-Tissue Expression) data bases, and 17 freshly paired BC and adjacent normal tissues were analyzed for mRNA levels of Cavin3 . Furthermore, cavin3 protein expression from 407 primary BC samples were assessed by immunohistochemistry (IHC) and measured by H-score. The clinical significance of cavin3 expression was explored by Kaplan-Meier analysis and the Cox regression method. In vitro biological assays were performed to elucidate the function and underlying mechanisms of cavin 3 in BC cell lines. Results Cavin3 mRNA was dramatically down-regulated in BC compared with the negative control. The median H-score of cavin3 protein by IHC was 50 (range 0-270). There were 232 (57%) and 175 (43%) cases scored as low (H-score≤50) and high (H-score >50) levels of cavin3, respectively. Low cavin3 was correlated with a higher T and N stage, and worse distant metastasis-free survival (DMFS) and overall survival (OS). Multivariate survival analysis revealed low cavin3 was an independent fact for worse DMFS. In BC cells, an overexpression of cavin3 could inhibit cell migration and invasion, and significantly decreased the level of p-Akt. Knockout of cavin3, meanwhile, promoted cell invasion ability and increased the level of p-AKT. Conclusion Cavin3 expression is significantly lower in BC and is correlated with distant metastasis and worse survival. Cavin3 functions as a metastasis suppressor via inhibiting the AKT pathway, suggesting cavin3 as a potential prognostic biomarker and a target for BC treatment.

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