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Cathepsin-D, a key protease in breast cancer, is up-regulated in obese mouse and human adipose tissue, and controls adipogenesis. : Cathepsin D, adipogenesis and obesity

Authors
  • Masson, Olivier
  • Prébois, Christine
  • Derocq, Danielle
  • Meulle, Aline
  • Dray, Cédric
  • Daviaud, Danielle
  • Quilliot, Didier
  • Valet, Philippe
  • Muller, Catherine
  • Liaudet-Coopman, Emmanuelle
Publication Date
Jan 01, 2011
Source
HAL-INRIA
Keywords
Language
English
License
Unknown
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Abstract

The aspartic protease cathepsin-D (cath-D) is overexpressed by human epithelial breast cancer cells and is closely correlated with poor prognosis in breast cancer. The adipocyte is one of the most prominent cell types in the tumor-microenvironment of breast cancer, and clinical studies have shown that obesity increases the incidence of breast cancer. Here, we provide the first evidence that cath-D expression is up-regulated in adipose tissue from obese human beings, as well as in adipocytes from the obese C57BI6/J mouse. Cath-D expression is also increased during human and mouse adipocyte differentiation. We show that cath-D silencing in 3T3-F442A murine preadipocytes leads to lipid-depleted cells after adipogenesis induction, and inhibits of the expression of PPARγ, HSL and aP2 adipocyte differentiation markers. Altogether, our findings demonstrate the key role of cath-D in the control of adipogenesis, and suggest that cath-D may be a novel target in obesity.

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