<bold><italic>Background:</italic></bold> Outcomes of neurobehavioral studies are invariably affected by the variations in the anxiety traits<bold><italic></italic></bold> of animals in the same species. Identifying those traits and categorizing them accordingly would improve the reproducibility of results, and reduce the variation in the results from different laboratories. <bold><italic>Purpose:</italic></bold> The present study was done to identify the possible groups among the normal population of outbred adult male Wistar rats. <bold><italic>Methods:</italic></bold> Anxiety traits were measured in elevated plus maze (EPM) test and open field test (OFT). The various anxiety responses from these tests were subjected to exploratory factor and hierarchical cluster analyses. Different clusters thus derived were compared with each other. <bold><italic>Results and Conclusion:</italic></bold> In exploratory factorial analysis, 2 components, that is, anxiety and activity were derived from the EPM and OFT parameters. Cluster analysis of EPM parameters classified the rats into 3 groups “high anxiety and low activity”, “medium anxiety and high activity”, and “low anxiety and medium activity”. Whereas, cluster analysis on OFT parameters identified one more group namely “low anxiety and high activity”. The rats which came under the clusters formed from the EPM and OFT parameters were not identical. Moreover, EPM and OFT may be measuring different aspects of anxiety.