Affordable Access

deepdyve-link
Publisher Website

Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid production.

Authors
  • Oliveira, Adriano H1
  • Ogrodowski, Cristiane C2
  • de Macedo, André C1
  • Santana, Maria Helena A2
  • Gonçalves, Luciana R B1
  • 1 Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil. , (Brazil)
  • 2 Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas, SP, Brazil. , (Brazil)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Dec 01, 2013
Volume
44
Issue
4
Pages
1097–1104
Identifiers
DOI: 10.1590/S1517-83822014005000017
PMID: 24688498
Source
Medline
Keywords
License
Unknown

Abstract

In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented with yeast extract or the conventional brain heart infusion medium. Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations. The hyaluronic acid concentration and viscosity increased from 0.15 g/L and 3.87 cP (no aeration or medium supplementation) to 1.76 g/L and 107 cP, when aeration (2 vvm) and 60 g/L of yeast extract were used. The results suggest the production of low-molecular weight hyaluronic acid oligomers instead of the high molecular weight polymer.

Report this publication

Statistics

Seen <100 times